ミラーの頃の化学研究、華やかりし頃の実験の話を聞くと何故かホッとする。今は洪水のような数の大衆研究者の下らない研究ばかりで欧米の学術雑誌を開くのも苦痛である。
Origin of life: Stanley Miller's forgotten experiments, analyzed
Date:June 25, 2014 sciencedaily.com
Source:Georgia Institute of Technology
Summary:
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Stanley Miller, the chemist whose landmark experiment published in 1953 showed how some of the molecules of life could have formed on a young Earth, left behind boxes of experimental samples that he never analyzed.
The first-ever analysis of some of Miller's old samples has revealed another way that important molecules could have formed on early Earth.
Images: 1) Stanley Miller in his UC San Diego lab in 1970/UCSD Archives.
The study discovered a path from simple to complex compounds amid Earth's prebiotic soup.
More than 4 billion years ago, amino acids could have been attached together, forming peptides. These peptides ultimately may have led to the proteins and enzymes necessary for life's biochemistry, as we know it.
In the new study, scientists analyzed samples from an experiment Miller performed in 1958.
To the reaction flask, Miller added a chemical that at the time wasn't widely thought to have been available on early Earth. The reaction had successfully formed peptides, the new study found. The new study also successfully replicated the experiment and explained why the reaction works.
"It was clear that the results from this old experiment weren't some sort of artifact. They were real,"
said Jeffrey Bada, distinguished professor of marine chemistry at the Scripps Institution of Oceanography at the UC San Diego. Bada was a former student and colleague of Miller's.
The study was supported by the Center for Chemical Evolution at the Georgia Institute of Technology, which is jointly supported by the National Science Foundation and the NASA Astrobiology Program.
The study was published online June 25 in the journal Angewandte Chemie International Edition. The work was primarily a collaboration between UC San Diego and the Georgia Institute of Technology in Atlanta. Eric Parker, the study's lead author, was an undergraduate student in Bada's laboratory and is now a graduate student at Georgia Tech.
Jeffrey Bada was Stanley Miller's second graduate student. The two were close and collaborated throughout Miller's career. After Miller suffered a severe stroke in 1999, Bada inherited boxes of experimental samples from Miller's lab. While sorting through the boxes, Bada saw "electric discharge sample" in Miller's handwriting on the outside of one box.
"I opened it up and inside were all these other little boxes,"
Bada said.
"I started looking at them, and realized they were from all his original experiments; the ones he did in 1953 that he wrote the famous paper in Science on, plus a whole assortment of others related to that. It's something that should rightfully end up in the Smithsonian."
The boxes of unanalyzed samples had been preserved and carefully marked, down to the page number where the experiment was described in Miller's laboratory notebooks.
The researchers verified that the contents of the box of samples were from an electric discharge experiment conducted with cyanamide in 1958 when Miller was at the Department of Biochemistry at the College of Physicians and Surgeons, Columbia University.
An electric discharge experiment simulates early Earth conditions using relatively simple starting materials. The reaction is ignited by a spark, simulating lightning, which was likely very common on the early Earth.
The 1958 reaction samples were analyzed by Parker and his current mentor, Facundo M. Fernández, a professor in the School of Chemistry and Biochemistry at Georgia Tech.
They conducted liquid chromatography- and mass spectrometry-based analyses and found that the reaction samples from 1958 contained peptides. Scientists from NASA's Johnson Space Center and Goddard Space Flight Center were also involved in the analysis.
The research team then set out to replicate the experiment. Parker designed a way to do the experiment using modern equipment and confirmed that the reaction created peptides.
"What we found were some of the same products of polymerization that we found in the original samples,"
Parker said.
"This corroborated the data that we collected from analyzing the original samples."
In the experiment from 1958, Stanley Miller had the idea to use the organic compound cyanamide in the reaction.
Scientists had previously thought that the reaction with cyanamide would work only in acidic conditions, which likely wasn't widely available on early Earth.
The new study showed that reactive intermediates produced during the synthesis of amino acids enhanced peptide formation under the basic conditions associated with the spark discharge experiment.
"What we've done is shown that you don't need acid conditions; you just need to have the intermediates involved in amino acid synthesis there, which is very reasonable,"
Bada said.
Why Miller added cyanamide to the reaction will probably never be known.
Bada can only speculate.
In 1958, Miller was at Columbia University in New York City. Researchers at both Columbia and the close-by Rockefeller Institute were at the center of studies on how to analyze and make peptides and proteins in the lab, which had been demonstrated for the first time in 1953 (the same year that Miller published his famous origin of life paper).
Perhaps while having coffee with colleagues someone suggested that cyanamide -- a chemical used in the production of pharmaceuticals -- might have been available on the early Earth and might help make peptides if added to Miller's reaction.
"Everybody who would have been there and could verify this is gone, so we're just left to scratch our heads and say 'how'd he get this idea before anyone else,'"
Bada said.
The latest study is part of an ongoing analysis of Stanley Miller's old experiments.
In 2008, the research team found samples from 1953 that showed a much more efficient synthesis than Stanley published in Science in 1953. In 2011, the researchers analyzed a 1958 experiment that used hydrogen sulfide as a gas in the electric discharge experiment.
The reactions produced a more diverse array of amino acids that had been synthesized in Miller's famous 1953 study. Eric Parker was the lead author on the 2011 study.
"It's been an amazing opportunity to work with a piece of scientific history,"
Parker said.
wired.com
A fresh look at forgotten vials from Stanley Miller’s primordial-soup-in-a-bottle experiments implies that volcanoes seeping hydrogen sulfide helped form some of life’s earliest ingredients.
Sulfur’s presence makes it possible to synthesize a greater variety of amino acids — the molecules that link to form protein chains — and gives nascent life a larger palette of chemicals from which to select.
“When you are analyzing old samples, you always hope in the back of your mind that you are going to find something really cool,”
says primary author Eric Parker, a graduate student now at Georgia Tech.
“It was a pleasant surprise to see such a large array of different amino acids and amines.”
In Miller’s classic experiments, dating from the early 1950s, electricity — standing in for lightning — zaps a few basic chemicals, water, methane, hydrogen and ammonia, to simulate the atmospheric conditions on Earth before life began.
Miller became famous for showing that simple chemicals could be combined with relative ease to form some of the building blocks of life.
This 1958 experiment, originally unpublished and revisited March 21 in the Proceedings of the National Academy of Sciences, marked Miller’s first inclusion of hydrogen sulfide, the chemical that makes rotten eggs smell and a prime component of volcanic gas emissions, in the primordial mix.
Using modern chemical analysis techniques, Parker’s team found 23 amino acids, seven other compounds and four amines, a breakdown product of amino acids.
This was far more than typical analysis of past samples, which yielded five to 10 amino acids. Several of the amino acids were synthesized for the first time, including methionine, a required building block for many proteins in animals, plants and fungi.
The newly identified collection of amino acids is also similar to those found in meteorites, meaning sulfur may have help assemble life’s ingredients off this planet, as well as on.
Sandra Pizzarello, an Arizona State University chemist who was not involved with the study, agreed with Parker’s chemical detective work. She noted, however, that all experiments seeking to synthesize prebiotic chemicals are limited.
Earth’s early atmospheric conditions aren’t known, and the reactions in this experiment could only have happened near a source of sulfur.
On a primordial Earth, that would have meant volcanoes. Whereas Miller originally focused on chemical reactions in the atmosphere, the primordial soup may have gathered in a volcanic bowl.
Volcanic origins were suggested by a 2008 reanalysis of another forgotten Miller experiment conducted by Jim Cleaves, a geochemist at the Carnegie Institution of Washington, and Scripps Institute of Oceanography biochemist Jeffrey Bada. Both are former students of Miller, and plan to revisit more of his old experiments.
“Miller was a real packrat. He didn’t throw anything out,”
said Cleaves, Miller’s final lab student and inheritor of the lab.
“Sitting on the shelf was this box, I thought, ‘I don’t know what these are, but I can’t bear to throw them out!’”
the constrained geometry of the pincer, which inhibits cyclometallation of the organic substituents on the donor sites at each end.